Collected via Leukapheresis Approximately 2 x 10^9 (2 billion) PBMCs Derived from two liters of whole blood Equivalent to four times the number of cells of a standard buffy coat. Volume of approximately 140 mL Further processing required to remove excess red blood cells Cell count and FACS analysis can be provided Blood/Cells can be stimulated prior to isolation Same day collection and shipment Repeat donors available Collected at our FDA licensed donor center under IRB approved protocols Donor informed consent approved by IRB

Our Other Immunology Products

Whole Blood Peripheral whole blood can be venously collected as full units (approximately 500 mL) or in individual vacutainers (up to 50 mL) Shipped fresh overnight for delivery the next morning Suitable for FACS analysis, PBMC preparation, further processing to cell subtypes, culture, etc. Blood can be drawn with the anticoagulant of your choice and the use of specific vacutainers can be requested Collected at our FDA licensed donor center under IRB approved protocols. Viral tested and found to be negative. Collected at our physicians sites under IRB approved protocols Donor informed consent approved by IRB BD® Cell Preparation Tubes (CPTs) Contained vacutainers system for the collection, cell separation, and transport of PBMC’s Cells isolated using density gradient fluid Approximately 5-10 x 10^6 (5-10 million) PBMCs per tube Available in Sodium Heparin and Sodium Citrate anticoagulant Tubes can be shipped spun or unspun Once isolated, gel barrier allows for easy transport of cells in the tube Cells can be easily poured off for further processing Collected at our FDA licensed donor center under IRB approved protocols Collected at our physicians sites under IRB approved protocols Donor informed consent approved by IRB

Buffy Coats Processed from a full unit (500 mL) of whole blood Approximately 5 x 10^8 (500 million) PBMCs Volume of approximately 50 mL Further processing required to remove excess red blood cells Same day collection and shipment Viral tested and found to be negative Collected at our FDA licensed donor center under IRB approved protocols Donor informed consent approved by IRB PBMCs PBMCs can be prepared from any of the above products Further processing performed, including RBC lysis, washing, and counting Can be shipped fresh overnight, cryopreserved in media, or frozen as a pellet Aliquoting by cell count Collected at our FDA licensed donor center uncer IRB approved protocols. Collected at our physicians sites under IRB approved protocols Donor informed consent approved by IRB

Disease States and Normal Controls

Bioreclamation continues to expand its proprietary Independent Review Board (IRB) approved network of specialty physician sites accessing high quality, consented clinical research samples and data for our clients. Samples, such as whole blood, CPTs and PBMCs can be collected by our physicians sites including:

Autoimmune/Rheumatology Endocrinology Oncology/Hematology

Cardiovascular Gastroenterology Pulmonology

Dermatology Neurology Virology

T: (516) 483-1196 | Email: immunology@bioreclamation.com